Fig. 3. In vivo blockade with allatotropin (AT)-antiserum of the AT-like peptide released by Malpighian tubules (MTs). Graphs represent the differences in the quantity of urine eliminated by insects injected with AT-antiserum (1:100), preadsorbed antiserum (1:100 plus 200 nmol of pure AT) or saline. (A) Urine eliminated at several time points after blood meal. (B) Accumulated volume of urine eliminated by groups with different treatments at several intervals after meal. (C) Comparison between treatments during the first 2 h after blood meal and during the next 22 h (2–24 h after blood intake). Each bar represents the mean ± s.e.m. of the volume of urine (N=4–6 samples per treatment). ^*Significant differences between insects injected with AT-antiserum and controls (saline and preadsorbed AT-antiserum). Data correspond to one of three experiments performed with similar results.