Fig. 2. Protein characteristics of K. marmoratus RhBG, RhCG1 and RhCG2. Amino acid compositions indicate that all three Rh proteins consist of hydrophobic and polar amino acids, and all three proteins are negatively charged at physiological pH. Hydropathy profiles (Kyte–Doolittle scale) indicate that high hydrophobicity regions are dispersed along all three sequences and these high hydrophobicity regions correspond to the predicted transmembrane domains (red blocks). All three Rh proteins have intracellular N- and C-terminals.