Fig. 3. Effects of chronic exposure of D. melanogaster larvae to dietary salicylate on (A) fluid secretion rate, (B) salicylate concentration in the secreted fluid and (C) transepithelial flux of salicylate across the main segment of isolated Malpighian tubules set up in the Ramsay assay. Each point represents the mean ± s.e.m. of 7–10 tubules. Solid and broken lines indicate the control and experimental group, respectively. Experimental larvae were raised for 10 days on a 10 mmol l^–1 salicylate-enriched diet. Control larvae were raised on a salicylate-free diet.