Fig. 7. Acute changes in killifish gill EDN1A, EDN1B and ECE1 mRNA levels as determined by quantitative RT-PCR. FWSW transfers are indicated by solid lines and solid circles and FWFW sham controls are represented by open circles and dotted lines (A,C,E). SWFW transfers are represented by solid lines and solid squares and SWSW sham controls by dotted lines and open squares (B,D,F). N=5 or 6 killifish per time and treatment and values are means ± s.e.m. Note the y axis is logarithmic. All values are normalized to L8 and standardized to chronic SW mRNA levels (see Fig. 9). (A,B) EDN1A mRNA levels, (C,D) EDN1B mRNA levels and (E,F) ECE1 mRNA levels. The asterisks indicate statistical significance (P<0.05) compared to sham value.