Fig. 3. Impacts of temperature and juvenile hormone (JH) on protein abundance. Blue and red boxes indicate 22° and 27°C treatments, respectively, across assay days (d) 5, 10 and 15. Colony workers (CW) were sampled directly from colonies and used to start assays on day 0. (A) Single replicate SDS-PAGE gel after Coomassie staining (10 µg protein per lane). Noteworthy proteins are indicated, which include unknown 100 and 50 kDa proteins as well as the caste regulatory Hex-1 and Hex-2 proteins that occur in the 80–85 kDa range. The lane labeled as M_r indicates molecular mass standards in kilodaltons (kDa). (B) Mean results for densitometric scans of three SDS-PAGE gels from three independent experimental replicates. Error bars represent s.e.m.