Fig. 4. Western blot showing impacts of temperature and juvenile hormone (JH) on JH-binding by various proteins. All colors and labeling correspond identically to that described for Fig. 3. Blots were probed with anti-JH antiserum, which directly enables identification of JH covalently bound to denatured proteins. Three key proteins were identified by the anti-JH, including the Hexamerin-1 (Hex-1) protein, an unknown 100 kDa protein and another unknown group of proteins in the 50 kDa range. Molecular mass markers were pre-stained and visible before immunodetection.