spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wood, C. M.
Right arrow Articles by Cameron, J. N.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wood, C. M.
Right arrow Articles by Cameron, J. N.
Journal of Experimental Biology 114,151-179 (1985)
Published by Company of Biologists 1985

Temperature and the Physiology of Intracellular and Extracellular Acid-Base Regulation in the Blue Crab Callinectes Sapidus

Chris M. Wood 1 and James N. Cameron 2

1 McMaster University, Department of Biology, Hamilton, Ontario, Canada L8S4K1
2 The University of Texas at Austin, Port Aransas Marine Laboratory, Port Aransas, Texas 78373, U.S.A.

To whom correspondence should be addressed.

The 14C-DMO/3H-inulin method for pHi was critically assessed in intact Callinectes and found to be reliable provided adequate equilibration time and significant radiolabel excretion were taken into account. An unusually high ‘mean whole body pHi’ (7.54 at 20°C compared with a pHa of 7.80) was due to a highly alkaline fluid compartment (pHi = 8.23) in the carapace. At 20°C the pHi of the heart was 7.35 and skeletal muscle pHi was 7.30, and there were small but consistent differences in the pHi of different muscle types. The change in pHa with temperature was -0.0151 u°C-1 between 10 and 30°C, slightly less than the slope for the neutral pH of water ({Delta}pN/{Delta}T ~= -0.0175 u °C-1). With data corrected to constant PiCoCo2 this was associated with a change in [HCO3-]a between 10 and 20°C ( -0.13 mequivl-1°C-1, constant PaCoCo2) and a change in PaCoCo2 between 20 and 30°C (+0.13Torr°C-1, constant [HCO3-]a). The disturbing effect of relatively small PiCOCO2 changes on this pattern was demonstrated. {Delta}pHi/{Delta}T slopes for all tissues except carapace were not significantly different from pHa/{Delta}T but generally lower than {Delta}pN/{Delta}T. The slope for the. carapace was very flat and greatly influenced the ‘mean whole body pHi’ slope (-0.0062u°C-1). In haemolymph in vitro at constant Picoco2 ‘passive’ {Delta}[HCO3-]/{Delta}T (-0.17mequivI -1°C-1) was comparable to that in vivo between 10 and 20°C, independent of absolute PCOCO2. and directly related to total protein concentration. Haemolymph non-bicarbonate buffer value ({beta}) was similarly related to protein, but increased with temperature. Crabs subjected to an acute 20->10°C shift showed initial overshoots of pHa and pHi associated with undershoot of PaCOCO2, all of which were corrected over 24 h as [HCO3-]a rose. During this period there was a significant net uptake

Key words: Temperature, intracellular pH, extracellular pH, crab.

Accepted on June 8, 1984




This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
S. A. Qadri, J. Camacho, H. Wang, J. R. Taylor, M. Grosell, and M. K. Worden
Temperature and acid-base balance in the American lobster Homarus americanus
J. Exp. Biol., April 1, 2007; 210(7): 1245 - 1254.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1985