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Journal of Experimental Biology 124,73-91 (1986)
Published by Company of Biologists 1986

Modulation of the Calcium Current of Molluscan Neurones by Neurotransmitters

H. M. GERSCHENFELD ¹, CONSTANCE HAMMOND ¹, and DANIÈLE PAUPARDIN-TRITSCH ¹

¹ Laboratoire de Neurobiologie, Ecole Normale Supérieure 46 Rue d'Ulm, 75230 Paris Cedex 05, France

In identified neurones of the snail Helix aspersa, the Ca²⁺ current can be modulated by biogenic amines and peptides in different ways.

1. A reversible increase of Ca²⁺ current is evoked by 1 µmoll^-1 serotonin (5-HT) in a group ofidentified snail ventral neurones (D6, D7, E1). This 5-HT-induced enhancement of Ca²⁺ current is probably mediaed by cyclic GMP. Neither cyclic AMP nor forskolin mimic the effect of 5-HT, but both the intracellular injection of cyclic GMP and the application of zaprinast (an inhibitor of a cyclic-GMP-dependent phosphodiesterase) induce a Ca²⁺ current increase. Moreover, when a maximal Ca²⁺ current increase is induced by cyclic GMP, 5-HT becomes ineffective and vice versa.

2. Decreases in the Ca²⁺ current can be generated by two mechanisms, (a) A reversible decrease of the Ca²⁺ current is evoked by both dopamine (1 µmoll^-1) and the neuropeptide FMRFamide (1 µmoll^-1) on different identified neurones. Intracellular injections of either cyclic AMP or cyclic GMP do not mimic the effect of dopamine or FMRFamide. Moreover, the intracellular injection of EGTA does not affect the Ca²⁺ current decrease induced by these transmitters, (b) An ‘irreversible’ decrease of the Ca²⁺ current of the D2 neurone is elicited by 5-20µmoll^-1 cholecystokinin octapeptide (CCK8). Intracellular Ca²⁺ plays a key role in the intracellular mediation of this effect since the intracellular injection of EGTA prevents the CCK 8-induced decrease of the Ca²⁺ current.

Key words: synapse, neurotransmission, serotonin, dopamine, FMRFamide, cholecystokinin

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