QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by TUFTS, B. L. Articles by BOUTILIER, R. G. Search for Related Content PubMed Articles by TUFTS, B. L. Articles by BOUTILIER, R. G.

Journal of Experimental Biology 156,139-151 (1991)
Published by Company of Biologists 1991

Interactions between Ion Exchange and Metabolism in Erythrocytes of the Rainbow Trout Oncorhynchus Mykiss

B. L. TUFTS ¹ and R. G. BOUTILIER ²

¹ Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1; Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
² Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1

Experiments were carried out to investigate the relationship between ion exchange and energy metabolism in rainbow trout erythrocytes in vitro. Under resting conditions, the sodium/potassium pump accounts for 20 % of the cellular energy budget. In the presence of the -adrenergic agonist isoproterenol, however, this increases to 43 %. Inhibition of the sodium/potassium pump with ouabain results in greater increases in erythrocyte water content and sodium and chloride concentrations and a greater decrease in erythrocyte potassium concentration following stimulation by isoproterenol. Moreover, the decrease in erythrocyte NTP levels observed following adrenergic stimulation does not occur when the sodium/potassium pump is inhibited with ouabain. Inhibition of the sodium/potassium pump also abolishes the increase in oxygen consumption by the cells which normally takes place following adrenergic stimulation. Finally, depletion of erythrocyte NTP levels by the sodium ionophore monensin or by previous incubation with nitrogen does not result in a significant increase in oxygen consumption. Thus, catecholamines appear to be crucial for the metabolic–membrane coupling that occurs following adrenergic stimulation in rainbow trout erythrocytes.

Key words: erythrocytes, trout, ion exchange, metabolism, Oncorhynchus mykiss

Accepted on November 16, 1990

This article has been cited by other articles:

				M. Phillips, C. Moyes, and B. Tufts The effects of cell ageing on metabolism in rainbow trout (Oncorhynchus mykiss) red blood cells J. Exp. Biol., January 3, 2000; 203(6): 1039 - 1045. [Abstract] [PDF]

				J. Pesquero, T. Roig, J. Sanchez, and J. Bermudez Na+-K+ pump and metabolic activities of trout erythrocytes during anoxia Am J Physiol Cell Physiol, July 1, 1999; 277(1): C29 - C34. [Abstract] [Full Text] [PDF]