|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 200, Issue 24 3109-3118, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
SU Sys, D Pellegrino, R Mazza, A Gattuso, LJ Andries and L Tota
Laboratory of Human Physiology and Pathophysiology, University of Antwerp (RUCA), 2020 Antwerp, Belgium.
Endocardial endothelial morphology and the physiological modulatory role of nitric oxide (NO) were studied in an in vitro preparation of the working intact heart of the frog Rana esculenta, which lacks coronary vasculature and is thus devoid of a coronary vascular endothelium. En face confocal scanning laser microscopy of samples of perfused fixed hearts demonstrated the presence of NO synthase as a cytoplasmic constituent of the endocardial endothelial cells. Stroke volume (as a measure of performance in paced frog hearts) and stroke work (as an index of systolic function) increased by approximately 5 % after inhibition of the NO-cGMP pathway with 10(-4 )mol l-1 NG-nitro-l-arginine methyl ester and by approximately 8 % after inhibition with 10(-6 )mol l-1 Methylene Blue. In contrast, stroke volume and stroke work decreased by approximately 22 % after activation of the NO-cGMP pathway with sodium nitroprusside (10(-4 )mol l-1), while 3-morpholinosydnonimine (5x10(-8) to 10(-5 )mol l-1) caused a decrease of between 15 and 30 % and 8-bromo-cGMP (10(-6 )mol l-1) a decrease of approximately 8 %. These responses were significantly attenuated after exposure of the ventricular luminal to Triton X-100 (0.05 %, 0.1 ml), which itself increased performance (by over 10 %) without detectable morphological changes. These results show that the endocardial endothelium of Rana esculenta produces amounts of NO sufficient to modulate ventricular performance.
This article has been cited by other articles:
|
|
 |
|
  R. Mazza, A. Gattuso, C. Mannarino, B. K. Brar, S. F. Barbieri, B. Tota, and S. K. Mahata Catestatin (chromogranin A344-364) is a novel cardiosuppressive agent: inhibition of isoproterenol and endothelin signaling in the frog heart Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H113 - H122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Eschenhagen and W. H. Zimmermann Engineering Myocardial Tissue Circ. Res., December 9, 2005; 97(12): 1220 - 1231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pellegrino, C. A. Palmerini, and B. Tota No hemoglobin but NO: the icefish (Chionodraco hamatus) heart as a paradigm J. Exp. Biol., October 15, 2004; 207(22): 3855 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Adler, H. Huang, Z. Wang, J. Conetta, E. Levee, X. Zhang, and T. H. Hintze Endocardial endothelium in the avascular frog heart: role for diffusion of NO in control of cardiac O2 consumption Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H14 - H21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Goumon, T. Angelone, F. Schoentgen, S. Chasserot-Golaz, B. Almas, M. M. Fukami, K. Langley, I. D. Welters, B. Tota, D. Aunis, et al. The Hippocampal Cholinergic Neurostimulating Peptide, the N-terminal Fragment of the Secreted Phosphatidylethanolamine-binding Protein, Possesses a New Biological Activity on Cardiac Physiology J. Biol. Chem., March 26, 2004; 279(13): 13054 - 13064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Imbrogno, M. C. Cerra, and B. Tota Angiotensin II-induced inotropism requires an endocardial endothelium-nitric oxide mechanism in the in-vitro heart of Anguilla anguilla J. Exp. Biol., August 1, 2003; 206(15): 2675 - 2684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gattuso, R. Mazza, D. Pellegrino, and B. Tota Endocardial endothelium mediates luminal ACh-NO signaling in isolated frog heart Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H633 - H641. [Abstract] [Full Text] [PDF]
|
|
