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Journal of Experimental Biology, Vol 203, Issue 19 3025-3031, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

The adrenergic volume changes of immature and mature rainbow trout (Oncorhynchus mykiss) erythrocytes

T Lecklin, A Tuominen and M Nikinmaa
Laboratory of Animal Physiology, Department of Biology, University of Turku, FIN-20014 Turku, Finland. tiilec@utu.fi.

In this study, we examined whether the adrenergic volume response of teleost erythrocytes is related to cell maturity. Rainbow trout (Oncorhynchus mykiss) were made anaemic by reducing their haematocrit to approximately 50 % of the original value. After 3-4 weeks, small, young erythrocytes were seen in the circulation. By measuring the volume distribution of blood samples from anaemic fish before and after noradrenaline stimulation (10 min, 10(-5)mol l(-1) final concentration), we were able to show that the volume response of young, immature erythrocytes to catecholamine stimulation was greater than that of mature erythrocytes. In addition, the membrane fluidity, measured using the steady-state fluorescence polarisation method, was greater in anaemic fish after 24 days of recovery from bleeding than in control fish. Since blood from anaemic fish contained a large fraction of immature erythrocytes, this result indicates that the fluidity of the membrane of immature erythrocytes is greater than that of mature erythrocytes.


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P. Koldkjaer, T. G. Pottinger, S. F. Perry, and A. R. Cossins
Seasonality of the red blood cell stress response in rainbow trout (Oncorhynchus mykiss)
J. Exp. Biol., January 15, 2004; 207(2): 357 - 367.
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