QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rescan, P.-Y. Articles by Fauconneau, B. Search for Related Content PubMed PubMed Citation Articles by Rescan, P.-Y. Articles by Fauconneau, B.

Red and white muscle development in the trout (Oncorhynchus mykiss) as shown by in situ hybridisation of fast and slow myosin heavy chain transcripts

Pierre-Yves Rescan^1,*, Bertrand Collet^1,2, Cecile Ralliere¹, Chantal Cauty¹, Jean-Marie Delalande^1,2, Geoffrey Goldspink² and Benoit Fauconneau¹

¹ SCRIBE-INRA, Campus de Beaulieu, 35042 Rennes, France and
² Department of Anatomy and Developmental Biology, Royal Free Hospital and University College Medical School, Rowland Hill Street, London NW3 2PF, UK

View larger version (75K): [in a new window]   Fig. 1. Comparison of the amino acid sequences of the carboxy-terminal part of the slow trout myosin heavy chain (MyHC) with MyHCs from other species. The accession numbers for the sequences are as follows: trout slow MyHC (AF211172), human alpha-MyHC (M21664), frog MyHC (AF097907), chicken slow MyHC (U85023). Identical residues are shaded.

View larger version (75K): [in a new window]   Fig. 2. Expression of slow (A–E) and fast (F–J) myosin heavy chain (MyHC) in trout embryo. (A) Stage 16 embryo (approximately 45 somites), lateral view. (B) Stage 15 embryo (35 somites), transverse section within a rostral somite pair. The label is restricted to the medial domain of the somite. (C) Stage 16 embryo, transverse section within a rostral somite pair. The slow-MyHC-positive cells have begun to move dorsally and ventrally. (D) Stage 17 embryo (50 somites), horizontal section. The slow-MyHC-positive cells progressively reach the lateral surface of the myotome. (E) Stage 22 embryo, transverse section. The slow-MyHC-positive cells are present in the outermost domains of the myotome. (F) Stage 14 embryo (30 somites), lateral view. The fast MyHC label is observed in the most rostral somites. (G) Stage 17 embryo, lateral view. The label progresses caudally as somites form. (H) Stage 16 embryo, dorsal view. The label is initially confined to medial cells in caudal somites (arrowhead) and spreads laterally as the somite matures rostrally. (I) Stage 15 embryo, transverse section within a somite of the trunk. The label is restricted to the medial domain of the somite. (J) Stage 17 embryo, horizontal section. The label fills most of the medial bulk of the somite. s, somite; n, notochord. Scale bars: A, 300µm; B,C,D,J, 25µm; E, 14µm; F, 250µm; G, 350µm; H, 200µm; I, 30µm.

View larger version (137K): [in a new window]   Fig. 3. Dual-colour in situ hybridisation for slow (dark blue) and fast (red) myosin heavy chain (MyHC). (A–E) Serial transverse sections of a stage 16 embryo (approximately 45 somites). (A) Posterior tail; fast and slow MyHC are still undetectable. (B) Middle tail; fast MyHC expression is evident in the medial domain of the somite. (C) Anterior tail; fast muscle cells now extend almost the full dorsoventral extent of the somite. (D) Posterior trunk (immediately anterior to the anal vent); the fast muscle cells fill the medial bulk of the somite. Slow muscle precursors are now evident and are mostly present in the medial domain of the somite (arrow). (E) Anterior trunk; the migrating slow muscle cells are now lateral to the differentiated fast muscle cells. (F) Transverse section through the middle trunk of an eyed-stage embryo (stage 20); the slow muscle cells have reached the outermost regions of the myotomes; other myogenic cells have differentiated into fast muscle. Scale bars, 45µm.