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Topography of different photoreceptor cell types in the larval retina of Atlantic halibut (Hippoglossus hippoglossus)

Jon Vidar Helvik1,*, Øyvind Drivenes1, Torstein Harboe2 and Hee-Chan Seo1,{ddagger}

1 Department of Molecular Biology, University of Bergen, PO Box 7800, N-5020 Bergen, Norway and
2 Institute of Marine Research, Austevoll Aquaculture Research Station, N-5392 Storebø, Norway
{ddagger} Present address: Sars Centre for Molecular Marine Biology, Thormøhlensgt. 55, N-5008 Bergen, Norway



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  		Fig. 1. Cellular expression of visual pigments and retinal mosaic expression in Atlantic halibut (Hippoglossus hippoglossus). The cellular identities of the different photoreceptors in the retina of Atlantic halibut larvae at the end of the yolk-sac period (40 days post-hatching) were investigated by in situ hybridisation analysis with RNA probes for the four different Atlantic halibut cone opsins: ultraviolet (A,B), blue (C,D), green (E,F) and red (G,H). A,C,E,G are radial sections, while B,D,F,H are tangential sections. Expression patterns in tangential sections from the central retina are with blue, green and red opsin probes, respectively, while tangential sections in the ventral retina are with ultraviolet opsin probe. Black arrows indicate photoreceptor cells expressing the respective opsins. The white arrow indicates photoreceptor cells that do not express green-sensitive opsin. ONL, outer nuclear layer; PE, pigment epithelium. Scale bars, 10µm.

 


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  		Fig. 2. Cellular distribution of various photoreceptor cells. Transverse sections (A,C,D,E) and sagittal sections (B,F) of Atlantic halibut retina (dorsal is up) at the end of the yolk sac stage (40 days post-hatching) are shown. The various photoreceptors were visualised by in situ hybridisation analysis with specific opsin probes: ultraviolet (A,B), blue (C), green (D), red (E) and rod (F). Arrows indicate the photoreceptor layer. Scale bars, 100µm.

 


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  		Fig. 3. Retinal location of the various cone opsins. Drawing of selected sections from a section series of Atlantic halibut larvae (40 days post-hatching). Four different halibut cone opsins probe are used. Cone distribution is indicated with colour code as follows: violet for ultraviolet- (A), blue for blue- (B), green for green- (C) and red for red sensitive-opsin. Scale bars, 0.5mm. D, dorsal; N, nasal; T, temporal; V, ventral.

 




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