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TEMPERATURE AND MYOGENIC FACTOR TRANSCRIPT LEVELS DURING EARLY DEVELOPMENT DETERMINES MUSCLE GROWTH POTENTIAL IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) AND SEA BASS (DICENTRARCHUS LABRAX)

D. WILKES¹, S. Q. XIE², N. C. STICKLAND², H. ALAMI-DURANTE³, M. KENTOURI⁴, A. STERIOTI⁵, G. KOUMOUNDOUROS⁵, B. FAUCONNEAU⁶ and G. GOLDSPINK^1,*

¹ Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, University of London, Rowland Hill Street, London NW3 2PF, UK
² Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
³ INRA-IFREMER, Station D Hydrobiologie 64310 Saint Pée Sur Nivelle, France
⁴ Biology Department, University of Crete, PO Box 1470, 71110 Iraklio, Crete, Greece
⁵ Institute of Marine Biology of Crete, PO Box 2214, 71003 Iraklio, Crete, Greece
⁶ INRA Fish Physiology, Campus de Beaulieu, 35042 Rennes, France
^* Author for correspondence (e-mail: g.goldspink{at}rfc.ucl.ac.uk )

View larger version (88K): [in a new window]   Fig. 1. In situ hybridisation of species-specific probes at different developmental stages. (A) Eyed stage trout hybridised with trout myogenin RNA, (B) eyed stage trout hybridised with trout MyoD RNA. (C) Sea bass embryo (30-somite stage) hybridised with sea bass MyHC RNA and (D) sea bass hatched embryo hybridised with sea bass MyHC RNA.

View larger version (27K): [in a new window]   Fig. 2. Comparison of northern blot signals revealed by conventional autoradiography (A and B) and by phosphoimaging (C). The phosphoimager image contains orders of magnitude more information than the autoradiograph and is not subject to signal saturation. Therefore the intensities of the bands on the image reflect the mRNA levels more accurately. Top panels, autoradiographs; bottom panels, stained gels.

View larger version (34K): [in a new window]   Fig. 3. Northern blot quantification of RNA levels for MyoD (A), myosin (B) and MyoHC (C) in duplicate at hatching and yolk sac resorption for trout larvae. Units are arbitrary signal strengths detected by phosphoimaging. 4, 8, 12, LAW4 and LAW8, temperature regime (see Materials and methods).

View larger version (28K): [in a new window]   Fig. 4. Northern blot quantification of RNA levels in duplicate at hatching and yolk sac resorption for sea bass larvae. Units are arbitrary signal strengths detected by phosphoimaging. 13, 15, 20, incubation temperature (°C).