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Antioxidant enzymes in the developing lungs of egg-laying and metamorphosing vertebrates

Adam P. Starrs, Sandra Orgeig^*, Christopher B. Daniels, Margaret Davies and Olga V. Lopatko

Department of Environmental Biology, Adelaide University, Adelaide SA 5005, Australia
Present address: Department of Clinical and Experimental Pharmacology, Adelaide University, Adelaide SA 5005, Australia

View larger version (20K): [in a new window]   Fig. 1. Changes in the activity of antioxidant enzymes catalase (A), SOD (B) and GPx (C) in the lungs of the bearded dragon Pogona vitticeps during late development, at hatch and in the adult. Values are units mg–1 protein and are means ± S.E.M. In all cases N=4–6, except in C, where ‘pip’ and ‘hatch’ data points are N=2 and N=1, respectively. Pairs of symbols (*, and ) indicate points that are significantly different from one another (P<0.05). In C, indicates that adult GPx activity is significantly higher than at any other stage of development.

View larger version (23K): [in a new window]   Fig. 2. Changes in the activity of antioxidant enzymes catalase (A), SOD (B) and GPx (C) in the lungs of the chicken Gallus gallus during late development and at hatch. Solid lines indicate changes under normoxic conditions and dashed lines indicate changes under hypoxic (17 % O2) conditions. Values are units mg–1 protein and are means ± S.E.M. In all cases N=4–6. Pairs of symbols (* and ) within the same experimental group indicate points that are significantly different from one another (P<0.05). indicates points for which there is a significant difference in antioxidant enzyme activity between normoxic and hypoxic conditions.

View larger version (19K): [in a new window]   Fig. 3. Changes in the activity of antioxidant enzymes expressed as units mg–1 protein in lung tissue of frogs at developmental stages 30–46. Lungs from 10–30 animals were pooled for each sample. Values are means, means ± range or means ± S.E.M., depending on the number of measurements per stage (N=1 for stages 30, 35, 38; N=2 for stages 32, 34, 42; N=3 for all other stages). Catalase activity (A) remained unchanged for stages 30–40 (y=0.8272x+43.811; r2=0.0997; N=10) and thereafter increased exponentially (y=4.6563e0.0705x; r2=0.7059; N=7; P<0.01). SOD activity (B) remained unchanged over the entire developmental period (y=0.0175x+5.7001; r2=0.0137; N=17). GPx activity (C) remained unchanged over the entire developmental period (y=5x10–5x+0.0255; r2=0.0011, N=17).