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Postnatal development and control of the pulmonary surfactant system in the tammar wallaby Macropus eugenii

Natalie J. Miller, Sandra Orgeig^*, Christopher B. Daniels and Russell V. Baudinette

Department of Environmental Biology, Adelaide University, Adelaide, SA 5005, Australia

View larger version (170K): [in a new window]   Fig. 1. Electron micrograph of alveolar type II cells lining the airspace in the lung tissue of Macropus eugenii at birth. Scale bar, 2 µm.

View larger version (125K): [in a new window]   Fig. 2. (A) Low-magnification electron micrograph of lung tissue from Macropus eugenii at birth. Scale bar, 2 µm. (B) Enlargement of the region highlighted in A, showing the distribution of SP-A within the cytoplasm and airspace and associated with lamellar bodies. Immunogold particles are indicated by arrows. Scale bar, 0.5 µm.

View larger version (123K): [in a new window]   Fig. 3. (A) Low-magnification electron micrograph of lung tissue from Macropus eugenii at birth. Scale bar, 5 µm. (B) Enlargement of the airspace region highlighted in black in A, showing the distribution of SP-B associated with extracellular surfactant. Immunogold particles are indicated by arrows. Scale bar, 0.5 µm. (C) Enlargement of the cell region highlighted in white in A, showing the distribution of SP-B within the cytoplasm and lamellar bodies. Immunogold particles are indicated by arrows. Scale bar, 0.5 µm.

View larger version (129K): [in a new window]   Fig. 4. (A) Low-magnification electron micrograph of lung tissue from Macropus eugenii at birth. Scale bar, 2 µm. (B) Enlargement of the airspace region highlighted in black in A, showing the distribution of SP-D associated with large aggregate extracellular surfactant. Immunogold particles are indicated by arrows. Scale bar, 1 µm. (C) Enlargement of the cell region highlighted in white in A, showing the distribution of SP-D associated with the periphery of the lamellar bodies. Immunogold particles are indicated by arrows. Scale bar, 0.5 µm.

View larger version (109K): [in a new window]   Fig. 5. (A) Type II cells (arrows) co-cultured with fibroblasts (asterisk) isolated from the lung of Macropus eugenii at 70 days of age. Scale bar, 10 µm. (B) Fluorescence microscopy of the same culture of type II cells isolated from the lung of Macropus eugenii seen in A, showing lamellar bodies fluorescing green after incubation with LysoTracker Green DND-26. Cells were examined using an excitation filter, BP460-490, and a barrier filter, BA510IF. Scale bar, 10 µm.

View larger version (10K): [in a new window]   Fig. 6. The basal phosphatidylcholine secretion of alveolar type II cells from 30- and 70-day-old Macropus eugenii. An asterisk indicates a significant decrease between 30 and 70 days (P<0.05). The sample size of each group is indicated above each column. Values are means ± S.E.M.

View larger version (22K): [in a new window]   Fig. 7. The effects of the hormones dexamethasone (DEX) (A), prolactin (PRL) (B) and triiodothyronine (T3) (C) on the amount of phosphatidylcholine secreted as a percentage of basal secretion from alveolar type II cells of 30- and 70-day-old Macropus eugenii. An asterisk indicates a significant increase above basal secretion (P<0.05). A double dagger indicates a significant decrease in agonist-stimulated secretion between 30- and 70-day-old joeys (P<0.05). The sample size of each group is indicated above each column. Values are means ± S.E.M.

View larger version (24K): [in a new window]   Fig. 8. The effects of the autonomic secretagogues isoproterenol (ISOP) (A) and carbamylcholine chloride (CARB) (B) on the amount of phosphatidylcholine secreted as a percentage of basal secretion from alveolar type II cells of 30- and 70-day-old Macropus eugenii. An asterisk indicates a significant increase above basal secretion (P<0.05). A double dagger indicates a significant decrease in agonist-stimulated secretion between 30- and 70-day-old joeys (P<0.05). The sample size of each group is indicated above each column. Values are means ± S.E.M.