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Amino acid modulation of in vivo intestinal zinc absorption in freshwater rainbow trout

Chris N. Glover^1,* and Christer Hogstrand^1,2

¹ Division of Life Sciences, King’s College, London, 150 Stamford Street, London SE1 9NN, UK and
² T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington, Kentucky, 40506-0225, USA

View larger version (20K): [in a new window]   Fig. 1. Profile of 65Zn(II) activity obtained from the efferent cannula following perfusion of the intestine with 50 µmol l–1 Zn(II) alone (control), or in solution with 100 mmol l–1 L-cysteine or L-histidine. Values are means ± S.E.M. (N=4–6 for amino acid treatments, N=10 for control). Slope values (3.98 for L-cysteine, 3.71 for L-histidine, and 2.71 for Zn(II) alone) were calculated over the linear phase of outflow (approx. 20–50 min).

View larger version (24K): [in a new window]   Fig. 2. Effect of (A) histidine, (B) cysteine or (C) taurine solutions upon retained and absorbed Zn(II) fractions following perfusion of in vivo cannulated intestine in combination with 50 µmol l–1 Zn(II). Retained Zn(II) fraction represents the fraction of perfused Zn(II) accumulated in the animal over the course of the 3-h perfusion (100 % of the control is equal to a retained Zn(II) fraction of 27 %), whereas absorbed Zn(II) fraction represents the proportion of perfused Zn(II) accumulated in post-epithelial compartments (100 % of the control is equal to an absorbed Zn(II) fraction of 11 %). Control data are consistent across all three plots. Values are means ± S.E.M. of 4–6 replicates for amino acid treatments, and of 10 replicates for control values. *Significant differences between control and treatment, between D- and L-stereoisomers, and between treatment concentrations were tested at P<0.05, ANOVA.

View larger version (24K): [in a new window]   Fig. 3. Effect of (A) histidine, (B) cysteine or (C) taurine solutions upon Zn(II) accumulation rate into epithelium (scrapings), subepithelium (intestinal tissue remaining following scraping) and post-intestinal (pooled blood and body) compartments following perfusion of in vivo cannulated intestine in combination with 50 µmol l–1 Zn(II). Values are means ± S.E.M. of 4–10 replicates. *Significant differences between control and treatment, between D- and L-stereoisomers, and between treatment concentrations were tested at P<0.05, ANOVA.

View larger version (18K): [in a new window]   Fig. 4. Effect of 100 mmol l–1 cysteine or taurine upon blood and body (carcass remaining after dissection) Zn(II) accumulation rates following perfusion of in vivo intestine in combination with 50 µmol l–1 Zn(II). Values are means ± S.E.M. of 4–10 replicates. *Significant differences tested at P<0.05, ANOVA.