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Transepithelial potential differences and Na⁺ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity

C. M. Luquet^1,*, U. Postel², J. Halperin^1,3, M. R. Urcola¹, R. Marques⁴ and D. Siebers²

¹ Department of Biological Sciences, FCEN University of Buenos Aires, Building II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina,
² Alfred Wegener Institute for Polar and Marine Research, Columbusstrasse, D-27568 Bremerhaven, Germany,
³ CONICET-Argentina and
⁴ Department of Inorganic Chemistry, FCEN University of Buenos Aires, Argentina

View larger version (12K): [in a new window]   Fig. 1. Transepithelial potential differences (TEPD) of gills of crabs acclimated to low salinity, perfused with different saline concentrations. TEPD of posterior gills increased significantly in absolute value at lower concentrations (P<0.001, N=6 for each gill). TEPD of gill 5 did not differ significantly from 0 mV (P>0.05).

View larger version (19K): [in a new window]   Fig. 2. Effects of ouabain and BaCl2 on the transepithelial potential difference (TEPD) of individual gills 6 from a crab acclimated to low salinity, perfused with 10  saline.

View larger version (15K): [in a new window]   Fig. 3. Unidirectional Na+ fluxes of gills 6 from crabs acclimated to low and high salinity, perfused with 20 and 38  saline, respectively. Ouabain (5 mmol l–1) added to the perfusate significantly inhibited both Jin of crabs acclimated to low salinity (P<0.05, N=5) and Jout of crabs acclimated to high salinity (P<0.001, N=4).

View larger version (10K): [in a new window]   Fig. 4. Transepithelial potential difference (TEPD) of gill 6 from crabs acclimated to high salinity, perfused at different saline concentrations (N=9).

View larger version (17K): [in a new window]   Fig. 5. Effects of ouabain, BaCl2 and choline chloride on the transepithelial potential difference (TEPD) of individual gills 6 from a crab acclimated to high salinity, perfused with 38  saline. Inhibition was almost irreversible.