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Differential expression of mitochondria-encoded genes in a hibernating mammal

Dustin S. Hittel and Kenneth B. Storey^*

Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6

View larger version (38K): [in a new window]   Fig. 1. Northern blot hybridization profiles and western blot profiles of CoxI (A) and CoxIV (B) genes and their protein products, respectively, in euthermic (E) and hibernating (H) ground squirrel kidneys. (C) Ratios of scanned band intensities for hibernating versus control values (means ± S.E.M., N=3 separately run northern and western blots; each blot contained RNA or protein, respectively, prepared from separate animals).

View larger version (54K): [in a new window]   Fig. 2. Northern blots (A) and analysis (B) of three mitochondrial encoded and two nuclear encoded genes whose protein products include subunits of complex IV (cytrochrome c oxidase) and V (ATP synthase) of the mitochondrial electron transport chain, in four tissues from euthermic and hibernating ground squirrels. Northern blots of total RNA (16 µg lane-1) were scanned, then band intensities quantified and normalized to their corresponding 28S ribosomal bands. The ratios of normalized band intensities were then calculated. Values are mean ± S.E.M., N=3 separately run northern blots; each blot contained RNA prepared from separate animals.

View larger version (72K): [in a new window]   Fig. 3. Northern blot of Cox1 transcript levels in euthermic (E), cold-adapted (C) and hibernating (H) 13-lined ground squirrel kidney (A), and an additional band (approx. 3.0 kb), which crossreacts with the Cox1 probe in hibernating kidney and brown adipose tissue (B).