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Temperature affects the ontogeny of sexually dimorphic cuticular hydrocarbons in Drosophila melanogaster

Fabrice Savarit¹ and Jean-François Ferveur^2,*

¹ LEEC, CNRS- FRE 2413, Université Paris 13, 93430 Villetaneuse, France
² CNRS-UMR 5548, Faculté des Sciences, Université de Bourgogne, 6 Boulevard Gabriel, 21000 Dijon, France

View larger version (21K): [in a new window]   Fig. 1. Production of predominant cuticular hydrocarbons in 4-day-old males as a function of the temporal induction of heat shock. Values are means of absolute amounts (ng) ± S.E.M. A single 1 h heat-shock pulse was induced between adult eclosion (AE) and 72 h AE. Four genotypes were used: (A) Cs; (B) hsp70-GAL4; Cs (hsp-Cs); (C) hsp70-GAL4 x UAS-lacZ (hsp-lacZ); (D) hsp70-GAL4 x UAS-tra (hsp-tra). For each genotype, data from non-heat-shocked flies (no HS) are shown on the right of each graph. For each data point, N=9-29 (except for hsp-Cs at 24 h; N=4). For abbreviations of hydrocarbons, see legend to Fig. 3.

View larger version (22K): [in a new window]   Fig. 2. Production of predominant cuticular hydrocarbons in 4-day-old females as a function of temporal induction of heat shock. For procedure and genotypes, see Fig. 1. For each data point, N=9-36. For abbreviations of hydrocarbons, see legend to Fig. 3.

View larger version (32K): [in a new window]   Fig. 3. Production of all detected cuticular hydrocarbons in 4- day-old flies after a single heat shock induced in adults 9 h old. Values are means of absolute amounts (ng) ± S.E.M. for heat-shocked (HS 9h; black bars) and non-heat-shocked (no HS; white bars) males (A—D) and females (E—F) of the hsp70-GAL4 x UAS-tra (hsp-tra) genotype. For genotypes, see Fig. 1. Hydrocarbon nomenclature: 9-T, cis 9-tricosene; 7-T, cis7-tricosene; 5-T, cis 5-tricosene; 23LIN, n-tricosane; 7,11PD, cis,cis7,11-pentacosadiene; 9-P, cis 9-pentacosene; 7-P, cis 7-pentacosene; 25LIN, n-pentacosane; 7,11HD, cis,cis7,11-heptacosadiene; 27BR, 2-methylhexacosane; 7-H, cis7-heptacosene; 27LIN, n-heptacosane; 7,11ND, cis,cis7,11-nonacosadiene; 29BR, 2-methyl-octacosane. The total of CHs shown here represents 88% of all detected CHs. For each genotype, N=10-20.

View larger version (25K): [in a new window]   Fig. 4. Ontogeny of cuticular hydrocarbons of various chain lengths during the first 2 days of imaginal life following a heat shock 3 h after AE. Hydrocarbons were pooled according to the length of their carbon chain (23C-33C). The hydrocarbons usually secreted by immature flies range from 29C-33C (broken lines), whereas the CHs produced by mature flies mostly have 23C-29C (solid lines). Experimental (+HS) and control (no HS) flies are males (A) and females (B) from the hsp70-GAL4 x UAS-tra (hsp-tra) genotype. Values are means of absolute amounts (ng) ± S.E.M. of pooled CHs after extraction in 6-48 h-old flies (age is given as h after AE). For each data point, N=8-10.