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Analysis of glycolytic enzyme co-localization in Drosophila flight muscle

David T. Sullivan^1,*, R. MacIntyre², N. Fuda¹, J. Fiori¹, J. Barrilla¹ and L. Ramizel¹

¹ Department of Biology, Syracuse University, Syracuse, NY 13224, USA
² Department of Genetics and Molecular Biology, Cornell University, Ithaca, NY 14853, USA

View larger version (129K): [in a new window]   Fig. 1. Glycerol-3-phosphate dehydrogenase (GPDH) localization in antibody-stained myofibrils. Myofibrils were isolated from wild-type Drosophila muscles and reacted with anti-GPDH serum and then reacted with fluorescein isothiocyanate-labeled goat anti-rabbit serum and visualized as described in Materials and methods.

View larger version (107K): [in a new window]   Fig. 2. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and aldolase localization in antibody-stained myofibrils, as in Fig. 1.

View larger version (69K): [in a new window]   Fig. 3. Triose phosphate isomerase (TPI), phosphoglycerate kinase (PGK) and phosphoglycerol mutase (PGLYM) localization in antibody-stained myofibrils, as in Fig. 1.

View larger version (102K): [in a new window]   Fig. 4. A comparison between (A) phalloidin and (B) anti-glycerol-3-phosphate dehydrogenase (GPDH) doubly stained myofibrils. Note that the thin Z-discs and the broader M-lines in the phalloidin-stained myofibril coincide with fainter anti-GPDH stained and brighter stained corresponding bands, respectively.

View larger version (75K): [in a new window]   Fig. 5. Myofibrils from wild-type Drosophila muscles incubated for 5 min in contraction buffer and stained for glycerol-3-phosphate dehydrogenase (GPDH). Note the reduced staining at the M-lines as compared with Fig. 1.

View larger version (90K): [in a new window]   Fig. 6. Myofibrils from wild-type Drosophila muscles incubated in contraction buffer for 20 min and simultaneously stained using anti-glycerol-3-phosphate dehydrogenase (GPDH) and phalloidin. Note the correspondence of the brightly fluorescent GPDH signal with the very faint, narrow dark bands on the phalloidin-stained myofibril.

View larger version (74K): [in a new window]   Fig. 7. Glycerol-3-phosphate dehydrogenase (GPDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and aldolase in antibody-stained myofibrils prepared from GPDH null mutant flies. Visualized as in Fig. 1. The exposure has been extended so that an image of the weakly fluorescent myofibril would be evident.

View larger version (76K): [in a new window]   Fig. 8. Triose phosphate isomerase (TPI), phosphoglycerate kinase (PGK) and phosphoglycerol mutase (PGLYM) in antibody-stained myofibrils prepared from glycerol-3-phosphate dehydrogenase (GPDH) null mutant flies. Visualized as in Fig. 1. The exposure has been extended so that an image of weakly fluorescent myofibril would be evident.