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First published online August 25, 2003

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Time course of the response of mitochondria from oxidative muscle during thermal acclimation of rainbow trout, Oncorhynchus mykiss

Patrice Bouchard and Helga Guderley^*

Département de Biologie, Université Laval, Québec, Canada, G1K 7P4

View larger version (41K): [in a new window]   Fig. 1. Time course of changes in maximal rates of pyruvate oxidation by mitochondria from oxidative muscle during warm and cold acclimation. Rates are expressed per mg mitochondrial protein, per nmol ADP-ATP translocase (ANT), per nmol cytochrome b (cyt. b) and per mg phospholipids. Data are means ± S.E.M. When rates at a given assay temperature during each acclimation experiment differ, they are followed by different letters (P0.05; ANOVA and Tukey's a posteriori test). *Denotes a significant difference between final cold rate (week 10) and initial cold rate (initial in warm acclimation). No differences were found between final warm rate (week 8) and initial warm rate (initial in cold acclimation). The number of individuals for each experiment is shown in Table 1. NA, not available.

View larger version (39K): [in a new window]   Fig. 2. Time course of changes in maximal rates of palmitoyl carnitine oxidation by mitochondria from oxidative muscle during warm and cold acclimation. Rates are expressed per mg mitochondrial protein, per nmol ADP-ATP translocase (ANT), per nmol cytochrome b (cyt. b) and per mg phospholipids. Data are means ± S.E.M. When rates at a given assay temperature during each acclimation experiment differ, they are followed by different letters (P0.05; ANOVA and Tukey's a posteriori test). *Denotes a significant difference between final cold rate (week 10) and initial cold rate (initial in warm acclimation). No differences were found between final warm rate (week 8) and initial warm rate (initial in cold acclimation). The number of individuals for each experiment is shown in Table 1.

View larger version (31K): [in a new window]   Fig. 3. Ratio of mitochondrial rates of pyruvate oxidation to maximal activities of cytochrome c oxidase (CCO) and citrate synthase (CS) during warm and cold acclimation. Values are expressed per mU CCO and per mU CS. Data are means ± S.E.M. When values at a given assay temperature during each acclimation experiment differ, they are followed by different letters (P0.05; ANOVA and Tukey's a posteriori test). The number of individuals in each experiment is shown in Table 1.

View larger version (38K): [in a new window]   Fig. 4. Time course of changes in state 4 and oligomycin-inhibited state 4 (state 4ol) rates of pyruvate and palmitoyl carnitine oxidation by mitochondria from oxidative muscle during warm and cold acclimation. Rates are expressed per mg mitochondrial protein. Data are means ± S.E.M. When rates at a given assay temperature during each acclimation experiment differ, they are followed by different letters (P0.05; ANOVA and Tukey's a posteriori test).

View larger version (29K): [in a new window]   Fig. 5. Muscle aerobic capacity and mitochondrial protein content during thermal acclimation. Data are means ± S.E.M. When values at a given assay temperature during each acclimation experiment differ, they are followed by different letters (P0.05; ANOVA and Tukey's a posteriori test).