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Involvement of Gq/11 in signal transduction in the mammalian vomeronasal organ

Kennedy S. Wekesa*, Stephanie Miller and Audrey Napier

Alabama State University, Biomedical Research and Training Programs, Montgomery, AL 36104-0271, USA



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  		Fig. 1. Identification of G-protein subunits in the vomeronasal organ (VNO) membranes. Each strip contained 20 µg of VNO membrane protein and was probed with a 1000-fold dilution of monospecific rabbit antisera against subunits of G-proteins, as indicated. The positions of molecular mass markers (MW) are indicated.

 


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  		Fig. 2. Immunohistochemical localization of G{alpha}o, G{alpha}i2 and G{alpha}q/11 to the microvillar surface of the vomeronasal organ. (A) A section stained with normal rabbit serum. (B) Section stained with a 250-fold dilution of antiserum against G{alpha}o. (C) Section stained with a 250-fold dilution of antiserum against G{alpha}i2. (D) Section stained with a 250-fold dilution of antiserum against G{alpha}q/11. Note the presence of staining within the neuroepithelium (arrowheads) for all G-protein antibodies (G{alpha}o, G{alpha}i2 and G{alpha}q/11). Scale bar, 100 µm. VNE, vomeronasal neuroepithelium.

 


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  		Fig. 3. The production of IP3 in female mouse vomeronasal organ (VNO) membranes by male mouse urine. Reactions were performed without stimuli (PBS), in the presence of 10% male urine, 100 µm GTP{gamma}S, 10% urine with GDPßs, 10% male urine with pertussis toxin (PTX) and 10% male urine with U73122. *Significantly different from control (PBS) value (P<0.05).

 




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