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First published online May 24, 2004
Journal of Experimental Biology 207, 2229-2236 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01007

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The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

K. Ocalewicz^1,*, I. Babiak^2,3, S. Dobosz⁴, J. Nowaczyk¹ and K. Goryczko⁵

¹ Department of Evolutionary Genetics, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
² Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
³ Department of Fisheries and Natural Sciences, Bodø Regional University, 8049 Bodø, Norway
⁴ Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, 83-330 Zukowo, Poland
⁵ Department of Fish Biology and Farming, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

View larger version (29K): [in a new window]   Fig. 1. Three karyotype patterns observed in androgenetic rainbow trout progeny from a single father: (A) 2n=58, (B) 2n=60 and (C) 2n=62. Scale bar, 10 µm.

View larger version (106K): [in a new window]   Fig. 2. Chromosomes of androgenetic rainbow trout (partial metaphase spreads) after DAPI staining. Arrows indicate chromosome fragments: (A) one DAPI-positive fragment (fish No. 10), (B) one DAPI-negative fragment (fish No. 14), (C) two DAPI-positive fragments (fish No. 12) and (D) three DAPI-negative fragments (fish No. 15). Scale bar, 10 µm.

View larger version (99K): [in a new window]   Fig. 3. Chromosomes of androgenetic rainbow trout after PRINS with telomere primers. Arrows indicate chromosome fragments in specimens No. 10 (A), 14 (B), 12 (C) and 15 (D) showing no hybridization signals. Arrowheads point to two out of three chromosome fragments in individual No. 15 having telomeres. Scale bar, 10 µm.

View larger version (92K): [in a new window]   Fig. 4. Chromosomes of (A) androgenetic and (B) normal rainbow trout after PRINS with telomere primers. Arrows indicate chromosomes possessing interstitial telomeric sites (ITS). (C) Karyotype of individual No. 12 (2n=58) with signals derived from telomeric sequences. Two pairs of chromosomes with ITS are shown by arrowheads. Scale bar, 10 µm.