QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online May 24, 2004
Journal of Experimental Biology 207, 2297-2304 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01021

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Clark, T. M. Articles by Remold, S. K. Search for Related Content PubMed PubMed Citation Articles by Clark, T. M. Articles by Remold, S. K.

pH tolerances and regulatory abilities of freshwater and euryhaline Aedine mosquito larvae

Thomas M. Clark^1,*, Benjamin J. Flis¹ and Susanna K. Remold²

¹ Department of Biological Sciences, Indiana University, South Bend, IN 46634-1700 USA
² Department of Ecology and Evolutionary Biology, Osborn Memorial Laboratory, Yale University, New Haven, CT 06520, USA

View larger version (10K): [in a new window]   Fig. 1. Hemolymph pH of larval Aedes aegypti (solid line) and Ochlerotatus taeniorhynchus (broken line) is highly regulated across the entire tolerable pH range from pH 4 to 11. Data are raw means ± S.E.M., sample size=6 replicates per species and pH value. **0.001<P<0.01; *0.01<P<0.05; NS, P>0.1 for t-tests comparing pH of the two species at each ambient pH.

View larger version (15K): [in a new window]   Fig. 2. Effects of rearing pH on life history parameters of larval Aedes aegypti (solid line) and Ochlerotatus taeniorhynchus (broken line) reared in buffered 3.5 g l–1 sea salt (Instant Ocean). Only larvae pupating successfully are shown. Effects of pH on (A) duration of the larval stage, (B) pupal wet mass and (C) growth rate. Males, filled symbols; females, open symbols. Data are raw means ± S.E.M.; sample size (A. aegypti)=148, sample size (O. taeniorhynchus)=71.

View larger version (15K): [in a new window]   Fig. 3. Effects of acclimation on pH tolerance of larval Aedes aegypti. (A) Survival times in extreme pH are increased by prior acclimation. Bars represent mean ± S.E.M. of survival time following transfer from acclimation pH to test pH. Sample sizes: 4 to 3, 32; 7 to 3, 24; 11 to 12, 27; 7 to 12, 10. (B) Acclimation to extreme pH does not influence survival at the other pH extreme. Bars represent the mean ± S.E.M. of the proportion of larvae surviving to pupation following acclimation at pH 4 or 11, then either maintained in the acclimation pH or transferred to the test pH. Samples sizes are four runs for all groups except 11 to 4, where only three runs were performed. (C) Transfer of acclimated larvae from one pH extreme to the other has little influence on the growth rate. Larvae were acclimatized to pH 4 or 11 for 3 days, then transferred to the other pH extreme. Males, filled bars; females, open bars. Sample sizes are (for males; females): 4 to 4 (8;8); 4 to 11 (14;6); 11 to 11 (9;15); 11 to 4 (10;10). Significance of comparison of transfers to same test pH are indicated for A and B; significance of comparisons within males or females from the same acclimation pH are shown for C. *P<0.05; NS, P>0.05.