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First published online July 26, 2004
Journal of Experimental Biology 207, 3025-3033 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01118

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Expression of hypothalamic arginine vasotocin gene in response to water deprivation and sex steroid administration in female Japanese quail

R. Seth^1,2, A. Köhler², R. Grossmann² and C. M. Chaturvedi^1,*

¹ Department of Zoology, Banaras Hindu University, Varanasi - 221 005 (UP), India
² Department Functional Genomics and Bioregulation, Institute for Animal Science (FAL), Mariensee - 31535, Neustadt am Rübenberg, Germany

View larger version (49K): [in a new window]   Fig. 7. Photomicrographs of quail brain showing negative control for (A) in situ hybridization. Brain sections were treated with RNase A prior to hybridization. (B) Brain section was not treated with AVT antiserum and served as a negative control for immunohistochemistry. Scale bars, 150 µm. PVN, paraventricular nuclei; 3V, third ventricle.

View larger version (20K): [in a new window]   Fig. 1. Effect of dehydration and sex steroid administration on (A) body mass and (B) plasma sodium ion concentration of Japanese quail. Values presented are means ± S.E.M. (N=10). *P<0.001 (significance of difference from control).

View larger version (21K): [in a new window]   Fig. 2. Effect of dehydration and sex steroid administration on (A) plasma osmolality and (B) plasma AVT concentration of Japanese quail. Values presented are means ± S.E.M. (N=10). *P<0.001 (significance of difference from control).

View larger version (52K): [in a new window]   Fig. 3. (A) Northern blot hybridization of hypothalamic RNA probed with fowl AVT cDNA and GAPDH. Each lane was loaded with 20 µg of total cellular RNA. C, control; WD, water deprived; TP, testosterone propionate treated; TP+WD, testosterone propionate treated + water deprived for last 2 days of injection; EB, estradiol benzoate treated; EB+WD, estradiol benzoate treated + water deprived for last 2 days of injection. (B) Hypothalamic AVT mRNA. Values are means ± S.E.M. (N=10). *P<0.05; **P<0.01; ***P<0.001 (significance of difference from control).

View larger version (69K): [in a new window]   Fig. 4. Photomicrographs of quail brain showing in situ hybridization of AVT mRNA (arrows), under dark field, in paraventricular nuclei on either side of the third ventricle (3V). Abbreviations as in Fig. 3. Scale bar, 150 µm.

View larger version (22K): [in a new window]   Fig. 5. Effect of dehydration and sex steroid administration on (A) number and (B) area of ir-AVT cell bodies in the paraventricular nucleus (PVN) region of the hypothalamus of Japanese quail. Values are means ± S.E.M. (N=10). *P<0.05; **P<0.01; ***P<0.001 (significance of difference from control). Abbreviations as in Fig. 3.

View larger version (115K): [in a new window]   Fig. 6. Immunohistochemistry of quail brain showing localization of ir-AVT (arrows) in paraventricular nuclei (PVN) on either side of the third ventricle (3V). Scale bar, 150 µm. Asterisks show empty neuronal cells. Abbreviations as in Fig. 3.