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First published online September 15, 2004
Journal of Experimental Biology 207, 3741-3748 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01210

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Transgenic salmon overexpressing growth hormone exhibit decreased myostatin transcript and protein expression

Steven B. Roberts^1,*, Linda A. R. McCauley^1,, Robert H. Devlin² and Frederick W. Goetz^1,

¹ Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA
² Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, BC V7V 1N6, Canada

View larger version (31K): [in a new window]   Fig. 1. Relative RNA abundance of MSTN1 and MSTN2 in brain, white muscle and red muscle. Values are means ± S.E.M. (N=8) for each fish group (transgenic, size control). For each graph, bars that do not share identical lowercase letters are significantly different when compared within fish groups (transgenic, size control) or tissue type (brain, white muscle, red muscle) at P0.05.

View larger version (34K): [in a new window]   Fig. 2. Northern blot of total RNA (10 µg/lane) from white and red muscle taken from five transgenic and five size-matched coho salmon probed with MSTN2. Corresponding 28s RNA expression is shown for each lane.

View larger version (25K): [in a new window]   Fig. 3. Relative abundance of MSTN1 and MSTN2 RNAs and MSTN immunoreactive protein (MIP; inset) in transgenic and control coho embryos taken prior to fertilization through hatching. Values are means ± S.E.M. (N=3). For RNA data, bars that do not share identical lowercase letters are significantly different when compared within fish group (transgenic, size-matched.) For protein data (inset), bars that do not share identical lowercase letters are significantly different when compared within fish group or within age at P0.05. The 42 kDa MIP was only detected at these three sampling ages shown, and the 14 kDa MIP was not detected in any embryo samples examined.

View larger version (19K): [in a new window]   Fig. 4. Relative protein abundance of MSTN immunoreactive protein (MIP) in white and red muscle from transgenic and size-control coho salmon. Abundance of the 42 kDa MIP (top) and the 14 kDa MIP (bottom). Values are means ± S.E.M. (N=8) at each sampling time. For each graph, bars that do not share identical lowercase letters are significantly different when compared separately within fish group (transgenic, size control) or tissue type (white muscle, red muscle) at P0.05.