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First published online November 5, 2004
Journal of Experimental Biology 207, 4205-4213 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01264

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Variation of crustacean hyperglycemic hormone (cHH) level in the eyestalk and haemolymph of the shrimp Palaemon elegans following stress

Simonetta Lorenzon, Paolo Edomi, Piero G. Giulianini, Romina Mettulio and Enrico A. Ferrero^*

BRAIN Center, Department of Biology, University of Trieste, via Giorgieri 7, I-34127 Trieste, Italy

View larger version (12K): [in a new window]   Fig. 1. Standard curve of known amounts of 6xHis-NencHHwt ELISA. The x axis represents the amount of peptide added per well (100 µl). Absorbance was read in a multiwell reader at 405 nm. Values of OD are means ± S.D. (N=4); correlation coefficients of the fitted curves are >0.99 for all the data (R=0.9937, S.D.=0.44, P<0.001).

View larger version (19K): [in a new window]   Fig. 10. Time course of glycaemia in the haemolymph of P. elegans after injection of two different concentrations of lipopolysaccharide and in the saline control and bled only control groups. Values of increment (see Materials and methods) are expressed as means ± S.D.

View larger version (18K): [in a new window]   Fig. 2. Time course of cHH in the eyestalk homogenates pooled from 10 P. elegans after exposure to different concentrations of Cu2+ and in the untreated control. Values are expressed as means ± S.D.

View larger version (14K): [in a new window]   Fig. 3. Time course of cHH in the hemolymph pooled from 10 P. elegans after exposure to different concentrations of Cu2+ and in the untreated control. Values are expressed as means ± S.D.

View larger version (20K): [in a new window]   Fig. 4. Time course of glycaemia in the haemolymph of P. elegans after exposure to different concentrations of Cu2+ and in the untreated control group. Values of increment (see Materials and methods) are expressed as means ± S.D.

View larger version (22K): [in a new window]   Fig. 5. Time course of cHH in the eyestalk homogenates pooled from 10 P. elegans after exposure to three different concentrations of Hg2+ and in the untreated control. Values are expressed as means ± S.D.

View larger version (20K): [in a new window]   Fig. 6. Time course of cHH in the haemolymph pooled from 10 P. elegans after exposure to three different concentrations of Hg2+ and in the untreated control. Values are expressed as means ± S.D.

View larger version (22K): [in a new window]   Fig. 7. Time course of glycaemia in the haemolymph of P. elegans after exposure to three different concentrations of Hg2+ and in the untreated control group. Values of increment (see Materials and methods) are expressed as means ± S.D.

View larger version (35K): [in a new window]   Fig. 8. Time course of cHH in the eyestalk homogenates pooled from 10 P. elegans after injection of two different concentrations of lipopolysaccharide and in the control. Values are expressed as means ± S.D.

View larger version (19K): [in a new window]   Fig. 9. Time course of cHH in the haemolymph pooled from 10 P. elegans after injection of two different concentrations of lipopolysaccharide and in the control. Values are expressed as means ± S.D.