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First published online December 22, 2003
Journal of Experimental Biology 207, 437-441 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00786

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Inhibition of the increased 17ß-estradiol-induced mast cell number by melatonin in the testis of the frog Rana esculenta, in vivo and in vitro

Gaia Izzo, Michela d'Istria, Ismene Serino and Sergio Minucci^*

Dipartimento di Medicina Sperimentale-Sezione di Fisiologia Umana e Funzioni Biologiche Integrate "F. Bottazzi", Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, via Costantinopoli 16, 80138 Napoli, Italy

View larger version (12K): [in a new window]   Fig. 1. In vivo effect of estradiol (E2), E2 administered in combination with melatonin, and melatonin (mel) alone on mast cell number (MCN) after 24 h or 48 h of treatment in testis of frogs captured during March. Values are expressed as means ± S.D. Different letters (a versus b) indicate significance at P<0.01.

View larger version (11K): [in a new window]   Fig. 2. In vitro effect of estradiol (E2; 10-6 mol l-1), E2 given in combination with melatonin (mel; 10-6 mol l-1), and melatonin alone (10-6 mol l-1) on mast cell number (MCN) after 6 h or 12 h ofincubation in testis of frogs captured in October. Values are expressed as means ± S.D. Different letters (a versus b) indicate significance at P<0.01.

View larger version (13K): [in a new window]   Fig. 3. In vivo effect of estradiol (E2) and E2 administered in combination with melatonin (mel) on mast cell number (MCN) in the frog Rana esculenta. All the animals were injected with E2 24 h before being sacrificed and were killed at 48 h, 72 h and on day 8 after the injection of melatonin or Krebs Ringer bicarbonate (KRB) buffer (control). Values are expressed as means ± S.D. Different letters indicate significance (a versus b, c, d, P<0.01; b versus d, e, P<0.01; b versus c, P<0.05; c versus d, e, P<0.01).

View larger version (11K): [in a new window]   Fig. 4. In vitro effect of estradiol (E2; 10-6 mol l-1) and E2 given in combination with melatonin (mel; 10-6 mol l-1, 10-9 mol l-1 or 10-12 mol l-1) on the mast cell number after 6 h of incubation in testis of frogs captured in October. Vertical bars indicate means ± S.D. Different letters (a versus b) indicate significance at P<0.01.