QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online March 9, 2004
Journal of Experimental Biology 207, 1335-1344 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00869

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, L.-Y. Articles by Hwang, P.-P. Search for Related Content PubMed PubMed Citation Articles by Lin, L.-Y. Articles by Hwang, P.-P.

Mitochondria-rich cell activity in the yolk-sac membrane of tilapia (Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels

Li-Yih Lin¹ and Pung-Pung Hwang^2,*

¹ Graduate Institute of Life Sciences, National Defense Medical Center, Nei-Hu, Taipei 114, Taiwan, ROC
² Institute of Zoology, Academia Sinica, Nankang, Taipei 11529, Taiwan, ROC

View larger version (46K): [in a new window]   Fig. 1. Confocal scanning of yolk-sac mitochondria-rich cells (MRCs) double-labeled with Con-A–Texas-Red (red) and Na+/K+-ATPase antiserum (second antibody conjugated with FITC; green) in larvae acclimated to high-Cl– medium (A,B) or low-Cl– medium (C,D). The x–y-plane (A,C) and z-plane (B,D) scanning images are shown. Magnification: 1000x. Scale bars: 15 µm (A,C); 10 µm (B,D).

View larger version (38K): [in a new window]   Fig. 2. Confocal scanning of yolk-sac mitochondria-rich cells (MRCs) double-labeled with Con-A–Texas-Red (red) and Na+/K+-ATPase antiserum (second antibody conjugated with FITC; green) in larvae. The densities of MRCs (A) and their exposed apical surfaces (B) were separately quantified, and the ratio of active MRCs over total MRCs was calculated as described in the Materials and methods. Scale bars, 50 µm.

View larger version (19K): [in a new window]   Fig. 3. Time course changes of active mitochondria-rich cells (MRCs) (A) and total MRCs (B) in yolk-sac membrane of tilapia larvae transferred from control water to low-Cl– or high-Cl– water for 48 h. Values are means ± S.D. (N=5). Different letters indicate significance at the P<0.05 level (one-way ANOVA followed by Tukey's pair-wise comparison).

View larger version (83K): [in a new window]   Fig. 4. Sequential observation of mitochondria-rich cells (MRCs) labeled with DASPEI in the yolk-sac membrane of tilapia larvae transferred from control to high-Cl– water for 0 h (A; in control water), 12 h (B) and 24 h (C). White numbers mark the cells that survived the acclimation, and yellow numbers mark the new cells generated after transfer. Magnification: 200x. Scale bars: 40 µm.

View larger version (19K): [in a new window]   Fig. 5. Ratio of surviving or newly generated mitochondria-rich cells (MRCs) in larvae transferred from control to low-Cl– or high-Cl– media for 24 h. Values are means ± S.D. (N=3). No significant difference was found between the three groups (one-way ANOVA followed by Tukey's pair-wise comparison).

View larger version (52K): [in a new window]   Fig. 6. Internalization of the apical membrane in mitochondria-rich cells (MRCs). Yolk-sac MRCs were double-labeled by Na+/K+-ATPase antiserum (second antibody conjugated with FITC; green) and Con-A–Texas-Red (red), which was internalized into the cytoplasm of MRCs (A). (B,C) Optical sections 3 µm and 6 µm beneath the apical surface. Magnification: 2000x. Scale bars: 15 µm.

View larger version (121K): [in a new window]   Fig. 7. FM1-43 staining and apical surface of mitochondria-rich cells (MRCs): (A–C) FM1-43 staining; (D–F) FM1-43, DASPEI and Con-A triple staining. See Results for detailed descriptions. Arrows represent the locations of MRCs. Magnification: 400x (A,C); 2000x (B); 1000x (D–F). Scale bars: 40 µm (A,C); 8 µm (B); 15 µm (D–F).