spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online May 1, 2006
Journal of Experimental Biology 209, 1848-1858 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02188
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bystriansky, J. S.
Right arrow Articles by Ballantyne, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bystriansky, J. S.
Right arrow Articles by Ballantyne, J. S.

Reciprocal expression of gill Na+/K+-ATPase {alpha}-subunit isoforms {alpha}1a and {alpha}1b during seawater acclimation of three salmonid fishes that vary in their salinity tolerance

J. S. Bystriansky1, J. G. Richards2, P. M. Schulte2 and J. S. Ballantyne1,*

1 Department of Integrative Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
2 Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada


Figure 1
View larger version (20K):

[in a new window]
  		Fig. 1. Plasma osmolality (mOsmol kg–1) of Arctic char, Atlantic salmon and rainbow trout acclimated to freshwater (control) or seawater (32{per thousand}) for up to 30 days. For clarity only one control time point (1 day freshwater, FW) is included for each species as it was not significantly different from the corresponding 30 day freshwater group. *Significantly different (P<0.05) from both freshwater control groups; asignificantly different (P<0.05) from corresponding 2, 4 and 10 day seawater groups, but not different from either freshwater control group; bsignificantly different (P<0.05) from corresponding 4 day seawater group, but not different from either freshwater control group. See text for interspecies comparisons. Values are means ± s.e.m.; sample size ranged from 5 to 8, and is listed in Results.

 

Figure 2
View larger version (25K):

[in a new window]
  		Fig. 2. Gill Na+/K+-ATPase activity of Arctic char, Atlantic salmon and rainbow trout acclimated to freshwater (control) or to seawater (32{per thousand}) for up to 30 days. *Activity is significantly higher (P<0.05) than in both freshwater control groups and the corresponding 1, 2 and 4 day seawater-acclimated group (dependent on species); aactivity is also significantly higher (P<0.05) than the corresponding 10 dayseawater group; {delta}freshwater Atlantic salmon had significantly higher activity than both Arctic char and rainbow trout freshwater control groups. Sample size for each group ranged from 5 to 8 and is listed in Results.

 

Figure 3
View larger version (18K):

[in a new window]
  		Fig. 3. Gill Na+/K+-ATPase (A) {alpha}1a and (B) {alpha}1b isoform mRNA levels in Arctic char acclimated to freshwater (control) or to seawater (32{per thousand}) for 30 days. mRNA level is relative to that of the control gene, elongation factor 1{alpha} (EF1{alpha}), and is normalized to the Arctic char freshwater 30 day (control) group (normalized to a value of one). *Significantly different (P<0.05) from both freshwater control points: asignificantly different (P<0.05) from the 1 day seawater group (B only). Sample size ranged from 5 to 8, and is listed in Results.

 

Figure 4
View larger version (16K):

[in a new window]
  		Fig. 4. Gill Na+/K+-ATPase (A) {alpha}1a and (B) {alpha}1b isoform mRNA levels in Atlantic salmon acclimated to freshwater (control) or to seawater (32{per thousand}) for 30 days. mRNA levels are relative to that of the control gene elongation factor 1{alpha} (EF1{alpha}) and normalized to the Arctic char freshwater 30 day (control) group (normalized to a value of one). *Significantly different (P<0.05) from both freshwater control points; asignificantly different (P<0.05) from the 4 day seawater group. Sample size equals 8 for all groups.

 

Figure 5
View larger version (17K):

[in a new window]
  		Fig. 5. Gill Na+/K+-ATPase (A) {alpha}1a and (B) {alpha}1b isoform mRNA levels in rainbow trout acclimated to freshwater (control) or to seawater (32{per thousand}) for 30 days. mRNA level is relative to that of the control gene, elongation factor 1{alpha} (EF1{alpha}) and normalized to the Arctic char freshwater 30 day (control) group (normalized to a value of one). *Significantly different (P<0.05) from both freshwater control points. Sample size ranged from 6 to 8, and is listed in Results.

 




© The Company of Biologists Ltd 2006