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First published online June 29, 2006
Journal of Experimental Biology 209, 2734-2738 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02279
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Effects of moderate and substantial hypoxia on erythropoietin levels in rainbow trout kidney and spleen

Jimmy C. C. Lai1, Izuru Kakuta2, Helen O. L. Mok1, Jodie L. Rummer3 and David Randall1,*

1 Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, SAR, China
2 Department of Biotechnology, Senshu University of Ishinomaki, 1 Shinmito, Minamisak, Ishinomaki, Miyagi 986-8580, Japan
3 Department of Zoology, University of British Columbia, Vancouver, BC, Canada


Figure 1
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  		Fig. 1. Erythropoietin (EPO) levels measured (via ELISA) for (A) rainbow trout kidney and (B) spleen during exposure of the fish to substantial hypoxia. Values are mean ±s.d., N=6. *Significantly different from control (P<0.05).

 

Figure 2
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  		Fig. 2. EPO levels measured (using ELISA) for rainbow trout kidney (A) and spleen (B) upon exposure to moderate hypoxia. Values are mean ± s.d., N=6. *Significantly different from control (P<0.05).

 

Figure 3
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  		Fig. 3. (A) Control (C1-C3) and 24 h hypoxia-exposed (H1-H3) rainbow trout renal EPO levels (obtained via western blot analysis) and (B) the relative EPO levels in control and hypoxic rainbow trout kidney. Values are mean ± s.d., N=3. *Significantly different from control (P<0.05). The positions of molecular mass markers are shown in A.

 

Figure 4
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  		Fig. 4. Spleen somatic index (SSI) for rainbow trout exposed to substantial (A) or moderate hypoxia (B). Values are mean ± s.d., N=6. *Significantly different from control (P<0.05).

 

Figure 5
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  		Fig. 5. Hb concentration for rainbow trout exposed to substantial (30% air saturation) hypoxia (A) and moderate (55% air saturation) hypoxia (B). Values are mean ± s.d., N=6. *Significantly different from control (P<0.05).

 

Figure 6
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  		Fig. 6. Plots of spleen somatic index against Hb concentration for rainbow trout exposed to short-term hypoxia (within 24 h) with the controls (A) and prolonged hypoxia (144-216 h) (B).

 




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