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First published online November 2, 2007
Journal of Experimental Biology 210, 4005-4015 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.010462
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Comparative thyroidology: thyroid gland location and iodothyronine dynamics in Mozambique tilapia (Oreochromis mossambicus Peters) and common carp (Cyprinus carpio L.)

Edwin J. W. Geven, Nha-Khanh Nguyen, Marcel van den Boogaart, F. A. Tom Spanings, Gert Flik and Peter H. M. Klaren*

Department of Animal Physiology, Faculty of Science, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands


Figure 1
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  		Fig. 1. Representative autoradiographs of 30 µm, whole-body cryosections of juvenile tilapia (A,B) and juvenile carp (C–F) exposed to 125I, and of tilapia (G,H) and carp (I,J) exposed to 125I and KClO4. Broken circles indicate the position of the subpharyngeal region (1), the gall bladder (2) and the kidney (3). Scale bars, 1 cm.

 

Figure 2
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  		Fig. 2. Tissue distribution of the radioactivity, shown as the percentage of the total dose injected in the fish per gram of tissue at 2, 24, 48 and 96 h after i.p. 125I injection in tilapia (A, N=6) and carp (B, N=6). Results are means ± s.d. One-way ANOVA was used for statistical evaluation. Asterisks show significant difference compared with the radioactivity level at 2 h (*P<0.05, **P<0.01, ***P<0.001).

 

Figure 3
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  		Fig. 3. Crossmon's staining of 7 µm thick sections of carp subpharyngeal region (A), head kidney (B) and kidney (C). Thyroxine immunohistochemistry on serial sections of carp subpharyngeal region (D), head kidney (E) and kidney (F). Scale bars indicate 200 µm.

 

Figure 4
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  		Fig. 4. bTSH (10 mIU ml–1)-mediated T4 release by carp subpharyngeal region (A, N=7), head kidney (B, N=7) and kidney (C, N=7). Results are means ± s.d. *P<0.05 (Student's t-test).

 

Figure 5
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  		Fig. 5. Radioactivity of iodide, thyroid hormone and thyroid hormone conjugates fractions, shown as the percentage of the total dose remaining in the fish at the time of sampling per gram of plasma, after Sephadex LH-20 chromatography at 2, 24, 48 and 96 h after i.p. 125I injection in tilapia (•, N=6) and carp ({circ}, N=6). Results are means ± s.d. One-way ANOVA was used for statistical evaluation. Significantly different levels of radioactivity compared with that at 2 h are indicated for tilapia (*P<0.05, **P<0.01, ***P<0.001) and carp (+P<0.05, ++P<0.01, +++P<0.001).

 

Figure 6
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  		Fig. 6. Radioactivity of iodide, thyroid hormone and thyroid hormone conjugates fractions, shown as the percentage of the total dose remaining in the fish at the time of sampling per gram of tissue, in the subpharyngeal region (A), kidney (B) and head kidney (C), after Sephadex LH-20 chromatography at 2, 24, 48 and 96 h after i.p. 125I injection in tilapia (•, N=6) and carp ({circ}, N=6). Results are means ± s.d. One-way ANOVA was used for statistical evaluation. Significantly different levels of radioactivity compared with that at 2 h are indicated for tilapia (*P<0.05, **P<0.01, ***P<0.001) and carp (+P<0.05, ++P<0.01, +++P<0.001).

 

Figure 7
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  		Fig. 7. Radioactivity of iodide, thyroid hormone and thyroid hormone conjugates fractions, shown as a percentage of the total dose remaining in the fish at the time of sampling per gram of tissue, in the bile (A) and the ambient water (B), following Sephadex LH-20 chromatography at 2, 24, 48 and 96 h after i.p. 125I injection in tilapia (•, N=6) and carp ({circ}, N=6). Results are means ± s.d. One-way ANOVA was used for statistical evaluation. Significantly different levels of radioactivity compared with that at 2 h are indicated for tilapia (**P<0.01, ***P<0.001) and carp (+P<0.05, ++P<0.01, +++P<0.001).

 




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