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Fig. 3. Light micrographs of 5 µm thick sections showing TonEBP immunoreactivity
(TonEBP-IR) in the renal cortex (A,B) and outer medulla (C,D) of control (A,C)
and 14-day water-deprived N. alexis (B,D). In A and B, black
arrowheads indicate the glomeruli. TonEBP-IR is weak in the renal cortex and
is evenly distributed between the cytoplasm and nucleus of control (A) and
14-day water-deprived mice (B). In C and D, black arrows indicate the nuclei
of epithelial cells lining the tubules that pass through the outer medulla,
and arrowheads indicate the tubule lumen. Moderate TonEBP-IR can be observed
in the nuclei and cytoplasm in the epithelial cells lining the renal tubules
of control N. alexis (C), but TonEBP-IR was more intense in the
nuclei than the cytoplasm of the epithelial cells in the outer medulla of
14-day water-deprived mice (D). Scale bars, 30 µm (A,B), 120 µm
(C,D).
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0.05). A significant increase
in TonEBP mRNA expression was shown in the kidney after 7 and 14 days of water
deprivation, but no change was detected after 3 days of water deprivation.
