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Figure 9


Fig. 9. (A) Representative recording of the transepithelial voltage (Vt) before and after the addition of 0.2 mmol l–1 diphenylamine-2-carboxylate (DPC) to the bathing saline. DPC briefly depolarises Vt but does not inhibit the spontaneous voltage oscillations. Nor does it prevent the reduction of Vt by 10 µmol l–1 Tenmo-ADFb (thin arrow; ADFb). (B) A representative recording of the basal membrane voltage (Vb) showing this is not affected by the addition of either 10 µmol l–1 Tenmo-ADFb or 1 nmol l–1 Achdo-KII (AKII). Thick arrows mark the point of insertion (downward) and withdrawal (upward) of the microelectrode, and horizontal bars show when peptides were present in the perfusate.





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