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Fig. 5. The presence of sucrose in the reward strongly affects the ability of honey
bees to recall the olfactory CS. Pollen foragers were conditioned with the APC
protocol, the antennal-stimulation-alone (AC) protocol or a protocol where the
antennae were stimulated with sucrose solution but the subject was fed water
(ACW). (A) The rate of acquisition was greatest for the APC protocol (lreg;
22=7.80, P=0.040)
(NAPC=69; NAC=58;
NACW=68). (B) When tested immediately after conditioning,
the level of response of subjects to the CS during the first recall trial was
not significantly different for the three protocols (
22=1.60, P=0.449) (NAPC=35;
NAC=29; NACW=31). After 24 h, the
response of the AC and ACW subjects was significantly lower than the responses
of the APC subjects (
22=9.21, P=0.010)
(NAPC=34; NAC=29;
NACW=37). *P<0.05.