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Fig. 8. Rate of T_S resetting measured by quantifying isothermal tracking in wild-type and hen-1 mutant worms. (A) Isothermal tracks made by wild-type worms grown overnight at 25°C and placed on a steep linear thermal gradient spanning 16–26°C across a 9 cm-diameter plate. Snapshots of the movement of animals on the gradient were digitized and overlaid such that their trajectories were visible. Isothermal tracks, artificially colored yellow, were defined as long vertical trajectories and emerged in a band of temperatures near the previous cultivation temperature. For comparison, a few white trajectories are also shown, representing worms that are not tracking isotherms in the same period of time. The T_S is quantified as the mean temperature of isothermal tracks exhibited by a certain population of worms. (B) Isothermal tracks exhibited by hen-1(tm501) worms that were grown overnight at 25°C and placed on steep linear thermal gradients (1°C/cm). (C–F) The time-course of T_S resetting of wild-type animals and hen-1(tm501) animals. In C and D, worms were cultivated overnight with bacterial food at 15°C or 25°C, then shifted to a plate containing food at 25°C or 15°C, respectively. In E and F, worms were grown overnight with bacterial food, then shifted to a new plate without food. The circles represent experimental data, and the broken lines depict an exponential fit of the wild-type data. At least two independent plates, 50 worms and 20 isothermal tracks were used for each data point.

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