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Files in this Data Supplement:
Fig. S1. Demonstration of antibody specificity. (A) Western blot of adult Drosophila protein preparations probed with NEP4 antiserum or control sera. While the application of preimmune serum or NEP4 antiserum preincubated with the antigen used for immunization does not yield any immunosignal, both isoforms are detected by the untreated antiserum (arrows). (B) Immunostainings of late staged Drosophila embryos. A distinct staining is visible only in embryos treated with the NEP4 antiserum (C). Control stainings with preimmune serum (A), secondary antibody only (B) or NEP4 antiserum preincubated with the antigen used for immunization (C) do not show any signal.
Fig. S2. Northern blot probed with antisense riboprobes raised against a coding region which is unique to nep4A. The bottom panel shows part of the radiant red stained gel with ribosomal RNA (rRNA) visible to demonstrate the loading of comparable RNA amounts (15.µg per lane). nep4A transcripts are detectable in embryonic (E), 3rd instar larval (L), pupal (P) and adult (A) RNA preparations.
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